background and overview[1]
3-phosphoglycerate kinase is a key enzyme in glycolysis. mitsubishi chemical resin widely exists in animals, plants and microorganisms. it catalyzes the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate, producing 1 molecule. atp has biological functions such as affecting dna replication and repair and stimulating viral rna synthesis, and is widely used in drug target design.
principle of 3-phosphoglycerate kinase (pgk) test kit[1]
3-phosphoglycerate kinase catalyzes 3-phosphoglycerate and atp to produce 1,3-di-n polyetheramine phosphoglycerate and adp, and 1,3-bisphosphoglycerate is removed from 3-phosphoglyceraldehyde. under the action of hydrogenase and nadh, 3-phosphoglyceraldehyde, nad and phosphoric acid are produced. the absorbance change at 340nm reflects the activity of 3-phosphoglycerate kinase.
apply[2]
cn201911421881.3 discloses a composite indigenous microbial domestication enzyme that effectively improves urban black and odorous water bodies and a method for treating urban black and odorous water bodies. the composite indigenous microbial domestication enzyme includes acetyl coa acyltransferase, 1-phosphofructokinase, and acetone acid phosphate dikinase, β-hydroxybutyryl-coa dehydrogenase, 3-phosphoglycerate kinase, nad-dependent β-hydroxybutyl-coa dehydrogenase and thiolase. a method for treating urban black and smelly water bodies includes the following steps: preparation and activation of a composite native microbial domestication enzyme; placing the activated composite native microbial domestication enzyme into the black and smelly water body for a period of time; and then constructing aquatic plants. the composite native microbial domestication enzyme of the present invention has the advantage of efficiently improving the quality of black and odorous sewage water; in addition, the treatment method of the present invention has the advantage of efficiently purifying the water body and sustainably adjusting and optimizing the water body environment.
